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Faculty of Science and Arts - Khulais
Document Details
Document Type
:
Article In Journal
Document Title
:
Purification and Characterization of two glycoproteins from the cattle tick Boophilus annulatus
تنقية وتعريف نوعين من الجليكوبروتينات من يرقات قراد البقر من نوع بؤفيلس أنيولاتس
Subject
:
Vaccine production
Document Language
:
English
Abstract
:
The present study was conducted to identify new target immunogenic molecules from the larval stage of the cattle tick, Boophilus annulatus (Acari: Ixodidae). Two specific larval glycoproteins (GLPs) were isolated by two-step affinity chromatography. The larval immunogens were first purified with CNBr-Sepharose coupled to rabbit anti-larval immunoglobulins, and the glycoproteins were then purified with Con-A Sepharose. These glycoproteins have molecular weights of approximately 32 and 15 kDa with isoelectric points between 6.8 and 7.2. Antibodies against the two GLPs, labeled I and II, were detected in the anti-whole tick, -whole larval, and -gut antigens through immunoblot analysis. These results suggest that these GLPs are good immunogens and can be useful in the vaccination of cattle against tick infestation
ISSN
:
1229845X
Journal Name
:
Journal of veterinary sceince
Volume
:
8
Issue Number
:
2
Publishing Year
:
1428 AH
2007 AD
Article Type
:
Article
Added Date
:
Tuesday, December 28, 2010
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
عمرو السيد الحكيم
Alhakim, Amr El Sayed
Researcher
Doctorate
Files
File Name
Type
Description
28323.pdf
pdf
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