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Document Type |
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Article In Journal |
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Document Title |
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Gamma interferon (IFN-gamma) and IFN-gamma-inducing cytokines interleukin-12 (IL-12) and IL-18 do not augment infection-stimulated Gamma interferon (IFN-gamma) and IFN-gamma-inducing cytokines interleukin-12 (IL-12) and IL-18 do not augment infection-stimulated |
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Document Language |
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English |
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Abstract |
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Periapical granulomas are induced by bacterial infection of the dental pulp and result in
destruction of the surrounding alveolar bone. In previous studies we have reported that
the bone resorption in this model is primarily mediated by macrophage-expressed
interleukin-1 (IL-1). The expression and activity of IL-1 is in turn modulated by a
network of Th1 and Th2 regulatory cytokines. In the present study, the functional roles
of the Th1 cytokine gamma interferon (IFN-gamma) and IFN-gamma-inducing
cytokines IL-12 and IL-18 were determined in a murine model of periapical bone
destruction. IL-12-/-, IL-18-/-, and IFN-gamma-/- mice were subjected to surgical pulp
exposure and infection with a mixture of four endodontic pathogens, and bone
destruction was determined by microcomputed tomography on day 21. The results
indicated that all IL-12-/-, IL-18-/-, and IFN-gamma-/- mice had similar infectionstimulated
bone resorption in vivo as wild-type control mice. Mice infused with
recombinant IL-12 also had resorption similar to controls. IFN-gamma-/- mice
exhibited significant elevations in IL-6, IL-10, IL-12, and tumor necrosis factor alpha in
lesions compared to wild-type mice, but these modulations had no net effect on IL-
1alpha levels. Recombinant IL-12, IL-18, and IFN-gamma individually failed to
consistently modulate macrophage IL-1alpha production in vitro. We conclude that, at
least individually, endogenous IL-12, IL-18, and IFN-gamma do not have a significant
effect on the pathogenesis of infection-stimulated bone resorption in vivo, suggesting
possible functional redundancy in proinflammatory pathways. |
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ISSN |
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0 |
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Journal Name |
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Clin Diagn Lab Immunol |
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Volume |
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11 |
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Issue Number |
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1 |
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Publishing Year |
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2004 AH
2004 AD |
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Article Type |
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Article |
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Added Date |
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Monday, May 5, 2008 |
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Researchers
| خالد بالطو | Balto, Khalid | Researcher | Doctorate | kbalto@kau.edu.sa |
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