Document Details

Document Type : Article In Journal 
Document Title :
Diacylglycerol-mediated Signaling of Neutrophils in Diabetics with or without Periodontal Disease
Diacylglycerol-mediated Signaling of Neutrophils in Diabetics with or without Periodontal Disease
 
Document Language : English 
Abstract : Diabetes mellitus is a metabolic disorder characterized by hyperglycemia. Diacylglycerols (DAG) are a group of second messenger molecules involved in the regulation of neutrophil functions. DAG mass can be modified in diabetic cells through direct activation of endogenous signaling pathways and receptor mediated events triggered by advanced glycation endproducts (AGE). OBJECTIVE: The aim of this study was to evaluate total DAG content, and DAG kinase (DAGK) activity in neutrophils of diabetic patients with periodontal disease. METHODS: Diabetic subjects with periodontitis (N=15) were compared to matched-healthy subjects (N=15) with no periodontal disease. Peripheral blood neutrophils were isolated and total lipid was extracted. DAG content was measured by its phosphorylation to radioactively labeled phosphatidic acid (PA). For DAGK activity, the cells were suspended in lysis buffer and the conversion of external DAG substrate into PA was measured by the uptake of radioactivity as above. RESULTS: Total DAG levels were significantly higher in diabetic neutrophils compared to healthy individuals (diabetes = 9, healthy = 9) (p 
ISSN : 0 
Journal Name : JDR 
Volume : 3082 
Issue Number : 2 
Publishing Year : 2003 AH
2003 AD 
Article Type : Article 
Added Date : Tuesday, May 20, 2008 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
ممدوح كريمةKarima, Mamdouh InvestigatorDoctoratemkarimah@kau.edu.sa

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