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Document Type |
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Thesis |
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Document Title |
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Hypolipidemic effect of Atorvastatin- Loaded- Nanoemulsion in Rats تأثير الأتورفاستاتين المحمل على مستحلب النانو على دهون الدم في الجرذان |
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Subject |
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Faculty of Sciences |
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Document Language |
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Arabic |
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Abstract |
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Atorvastatin (ATV) is an anticholesteremic agent, HMG-CoA reductase inhibitor approved for clinical use as a lipid lowering agent. ATV, the world’s best-selling drug is frequently associated with implications of the low solubility and poor dissolution which affects its rate of absorption, resulting in a low oral bioavailability and have some complications. Nanoemulsion (NE) is an advanced mode of drug delivery system that has been developed for improving the delivery of therapeutic agents. The aim of this study was to develop and assess the ability of the NE in enhancing ATV bioavailability and minimizing its side effects in hyperlipidemic rats. Thirty five rats were divided into seven groups at which group has five rats. Hyperlipidemia was induced by feeding the animals high fat diet for 3 months and half. The antihyperlipidemic activity of 10 and 20 mg/kg of ATV loaded in two different delivery system, either loaded in nanoemulsion (10% and 20% ATV-LNE) or in water (10% and 20% ATV-sol) were investigated. The effectiveness of ATV formulations were assessed by measuring the change in body weight, serum and plasma biochemical parameters (lipid profile, glucose, insulin, liver function, kidney function, oxidative stress marker ( and studying the histopathological changes of the liver and kidney tissues. The physical characteristics of NE were determined by the Zetasizer (the z-average diameter and zeta potential). The 20% ATV-LNE has the smallest nanoparticles (38.12 ± 6.71nm) whereas it has the largest zeta negative potential of -26.8 ± 4.16mV. The serum biochemical results and the histopathological examination revealed that treatment with 20% ATV-LNE improved the lipid profile by significantly increasing HDL and decreasing cholesterol and low density lipoprotein. Also, serum glucose level was reduced in both 10 and 20% ATV-LNE compared to solution formulations. In addition, Alkaline Phosphatase (ALP) levels in all NE treated groups were lower than solution treated groups. In conclusion, the NE formulas have the potential to improve the bioavailability and efficacy of ATV and reduce its side effects. |
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Supervisor |
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Dr. Khadijah Saeed Balamash |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1438 AH
2017 AD |
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Added Date |
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Wednesday, October 18, 2017 |
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Researchers
| منى صالح الديني | Al-ddyni, Mona Saleh | Researcher | Master | |
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