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Document Type |
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Thesis |
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Document Title |
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Light and Watering Effects in Some Pharmaceutical Compounds Accumulation in Calotropis procera تأثير الضوء والري على تراكم المركبات الدوائية في نبات العشار |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Our study aim to identify the suitable day light period for high accumulation of pharmaceutical compounds in Calotropis procera in addition the effect of irrigation in concentration of these compounds. In this study, using UPLC/MS/MS separation, concentration change in very important five of glycosides - which have medical affects- were detected and observed according the environment conditions (light- irrigation). These compounds are Calotropagenin, Calotoxin, α-amyrene, β-sitosterol and Uzarigeni. Also, the suitable time for these pharmaceutical compounds accumulation in high concentrations was detected and we found that it is variable depend on compound types. Calotropagenin1 concentration was highly significant accumulation at down after irrigation (iD) and the lowest concentration was in midday before irrigation (M) and pre-dusk before irrigation (PD). Calotropagenin2 concentration was highly significant accumulation at midday after irrigation (iM), but it was disappeared in down before and after irrigation (D and iD). Uzarigenin di glycoside concentration was highly significant accumulation at midday before irrigation (M) but was lowest in pre-dusk after irrigation (iPD). However, Uzarigenin was highly significant accumulation in all time except dawn before irrigation (D). Also, α-amyrene, Uzarigenin was highly significant accumulation in all time except dawn after irrigation (iD) was a lowest. β-sitosterol was highly significant accumulation in dawn before and after irrigation (D and iD), however, it was not shown in midday after irrigation (iM) Calotoxin1 showed only in pre-dusk before irrigation (PD). Using bioinformatics tools and RNA-seq data, we successes to identify four genes related to pharmaceutical compound production in Calotropis plant; flavonol oxygenase (accession no. KU997644 (, oxysterol-binding protein (accession no. KU997645). |
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Supervisor |
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Dr. Ahmed Mohamed Mohamed Emara |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1440 AH
2019 AD |
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Co-Supervisor |
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Dr. Hani Mohammed Ali |
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Added Date |
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Wednesday, May 29, 2019 |
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Researchers
| سعيد عبد الله باعباد | Baabad, Saeed Abdullah | Researcher | Master | |
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