Document Details
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Document Type |
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Thesis |
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Document Title |
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Genetic Analysis in Thyroiditis and Papillary Thyroid Carcinomas with or without Coexisting Thyroiditis التحليل الجيني لالتهاب الغدة الدرقية وسرطان الغدة الدرقية الحليمي مع أو بدون الإصابة بالتهاب الغدة الدرقية |
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Subject |
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Faculty of Applied Medical Sciences |
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Document Language |
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Arabic |
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Abstract |
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Background: Autoimmune thyroiditis (AT), also known as Hashimoto's thyroiditis (HT) or chronic lymphocytic thyroiditis, is present in the background of around 30% of papillary thyroid carcinomas (PTCs). Thyroid carcinoma is the second most common thyroid malignancy in female in Saudi Arabia and affects around 9% of the population. On the molecular expression level, the predisposition of this autoimmune disease is not entirely understood.
Materials and methods: For this study, we analyzed retrospectively microarray expression profiles of 33 thyroid lesions including 13 HT cases, 6 micro PTCs (mPTCs, tumor size ≤ 1 cm) and 6 PTCs with coexisting HT and 8 PTCs without coexisting HT. The samples were processed and hybridized to HuGene 1.0 ST microarrays (Thermo Fisher Scientific, Waltham, MA). Using a software program, differentially expressed gene (DEG) sets were computed by utilizing a false discovery rate adjusted p-value ≤ 0.05 and a fold change > 2. For control samples, electronic files containing microarray files from normal thyroid samples were retrieved from a publicly accessible source.
Results: Approximately 400 DEGs distinguish HT from thyroid normal (TN) while more than 70 DEGs were shared between HT and PTCs with coexisting thyroiditis. More than 1000 DEGs were shared between HT and micro PTC (mPTCs), PTC with HT, and PTCs without HT, The DEGs shared between HT and PTCs with HT include a number of upregulated immunogenic genes, such as immunoglobulin kappa variable 1D-33 (IGKV1D-33), and interleukin 2 receptor gamma (IL2RG). Other upregulated genes were, e.g., small nucleolar RNA SNORA52 (SNORA52) and matrix metalloproteinase 9 (MMP9). Comparably downregulated genes include, e.g., acyl-CoA dehydrogenase (ACAD), and EYA transcriptional coactivator and phosphatase 4 (EYA4).
Conclusion: This is one of the first microarray studies that compares microarray expression profiles between HT and PTC. It gives a general view of DEGs common in both HT and PTC and supports the detection of molecular biomarkers which are of further interest for basic genetic and translational investigations in HT and thyroid cancer emerging on existing HT. |
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Supervisor |
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Dr. Hans Juergen Schulten |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1440 AH
2019 AD |
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Added Date |
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Wednesday, July 31, 2019 |
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Researchers
| عهود فيصل سبحي | Subhi, Ohoud Faisal | Researcher | Master | |
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