Document Details

Document Type : Thesis 
Document Title :
GENE EXPRESSION STUDY ON MICE SPLEEN CELLS DURING THE DEVELOPMENT OF CANCER AND EXPOSURE TO THE PROBIOTIC BACTERIA
دراسة التعبير الجيني لخلايا طحال الفئران أثناء تطور السرطان والتعرض لبكتيريا البروبيوتيك
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Colon cancer is one of the most common type of cancer, with an estimated incidence of about 11% of all cases cancer around the world. Notably, the early stages of tumors can be controlled via the anticancer effects of the spleen. On the other hand, in advanced stage of tumors, these antitumor effects are diminished or even absent. Interestingly, certain probiotics have been associated with cancer prevention as well; they module the anticancer effects by releasing cytokines to affect the innate and adaptive immune responses. Thus, to explore the potential antitumor effects of Streptococcus (S) thermophilus bacteria on Swiss mice spleens during induction of colon cancer, we categorized the mice into four groups: untreated negative controls (NC group), positive controls that were injected with azoxymethane (AOM) to induce colon cancer and the estimated dose was 10 mg/kg body weight for eight weeks (PC group), along with two different mice groups injected with AOM and orally administered with either 109 colony forming units (CFU) of Lactobacillus (L) plantarum (LPA group) or 108 CFU of S. thermophilus (STA group) for ten weeks. Then, we evaluated the transcription levels of spleen’s immune markers. We observed that the expression levels of TLR-1, TLR-2, IL-1β, TNFα, IFN-γ, IL-2, IL-4, and IL-10 were significantly elevated in week 4 in the spleen cells of the STA group when compared with their corresponding levels in the LPA and PC groups. This increase in the immunomarker transcription levels in the STA spleen cells diminished significantly in weeks 8 and 10 when compared with their corresponding levels in the LPA and PC groups. Thus, this study reports a withdrawal of antitumor effects in the later stages of cancer due to continuous exposure to S. thermophilus in the mice spleen cells during the early stages of colon cancer development; although, we did observe a very limited immune enhancement in the early stages of cancer. 
Supervisor : Dr. Sahar Ragab Mohamed El Hadad 
Thesis Type : Master Thesis 
Publishing Year : 1443 AH
2022 AD 
Added Date : Monday, January 16, 2023 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
ايمان مهاوش الرويليAl - Rowily, Eman MhawishResearcherMaster 

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