Document Details

Document Type : Thesis 
Document Title :
Imatinib in combination with anti-CD47 exerts synergetic anti-leukemia effect in chronic myeloid leukemia
تأثير دمج الايمتينيب مع anti-CD47 على خلايا سرطان الدم النخاعي المزمن
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Chronic myeloid leukemia is cancer that characterized by myeloid cells accumulation in peripheral circulation and unregulated bone marrow development. Imatinib Mesylate -5mg- is considered as the first line of treatment. Cancer immunotherapy such as monoclonal antibody currently offer a better option with less toxicity comparing to small molecules treatment. One of the fundamental features of cancer cells is immune evasion. Immune evasion occurs when CD47 -a transmembrane protein- that binds to SIRP-alpha and release (don’t eat me signal). Cancer cells utilize from this interaction to grow uncontrollably. Blocking CD47 with monoclonal antibody will enable immune system to recognize and destroy cancer cells. Reducing this dose without effecting the treatment efficacy was conducted by combining lower dose of IM with anti-CD47mAb clone BRIC126. Blast CML K562 cell line was used in the study. Cells were cultured and divided into 6 groups including (control group-BRIC126-5µM and 2.5µM of imatinib). Then levels of (GAB-2, SHC-1, PTPN6) which are proteins that implicated in cell signaling pathways was measured through ELISA. As well as CYQUANT cell proliferation assay to determine the amount of DNA and the ability of cells to proliferate after the treatment. Results indicated that with lowering the dose of IM by half and combining it with BRIC126, cells are proceeding to apoptotic pathways. Additionally, proliferation rate was controlled in all studied groups. In vitro findings indicated that this combination may be successful therapy. 
Supervisor : Dr. Wesam Abdulaal 
Thesis Type : Master Thesis 
Publishing Year : 1445 AH
2023 AD 
Added Date : Wednesday, November 1, 2023 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
بشرى مفرح عسيريAsiri, Bushra ResearcherMaster 

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 49480.pdf pdf 

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